Breast cancer remains one of the most common cancers worldwide, prompting researchers to investigate new plant-based compounds that may influence tumor growth. In this laboratory study, scientists examined a dichloromethane extract from Cannabis sativa to understand how its natural phytochemicals affect human breast cancer cell lines (MDA-MB-231 and MCF-7). The research focused on whether compounds found in cannabis could influence oxidative stress, cell survival, and cancer cell invasion, which are key processes involved in tumor progression. (PMC)

The study found that the cannabis extract reduced cancer cell viability in a dose-dependent manner and triggered apoptosis, or programmed cell death. Researchers observed activation of proteins associated with cell death pathways and changes in oxidative stress markers within the cancer cells. In addition, the extract significantly decreased the activity of molecules such as MMP-1, MMP-9, and TGF-β, which are linked to cancer cell migration and metastasis. These findings suggest the extract may limit both tumor growth and the ability of cancer cells to spread under experimental conditions. (PubMed)

Overall, the researchers concluded that bioactive compounds in Cannabis sativa—including cannabinoids and other plant molecules—may influence multiple biological pathways involved in breast cancer development, including oxidative stress regulation, apoptosis signaling, and anti-invasive mechanisms. Because the research was conducted in cell cultures rather than in humans, further animal studies and clinical trials are needed to determine safety and real-world therapeutic potential. (PMC)

Source

Ngnameko CR, Manjia JN, Matsabisa MG. Potential Anticancer Effect of Cannabis sativa L. Dichloromethane Extract Through Oxidative Stress‑Related Pathways and the Inhibition of the Migration and Invasiveness of Human Breast Cancer Cells (MDA‑MB‑231 and MCF‑7) (December 2025) Available through the National Library of Medicine (NIH): https://pmc.ncbi.nlm.nih.gov/articles/PMC12785486/